The AP2 domain class transcription factor, GRAS family transcription factors, and cyclin-dependent kinase were among the highly expressed meta-genes identified in the signature.
![reliability workbench fault tree batch analysis reliability workbench fault tree batch analysis](https://blog.isograph.com/wp-content/uploads/2017/07/Monopropellant-propulsion2-2.png)
The AM inoculation signature obtained was able to differentiate efficiently between AM inoculated and non-inoculated samples.
![reliability workbench fault tree batch analysis reliability workbench fault tree batch analysis](https://img.informer.com/p8/reliability-workbench-v9.1-main-window-display.png)
Feature weighting models also documented that this signature is independent from study (batch) effect. In total, 73 genes selected by machine learning models were up-regulated in response to AM (Z-value difference > 0.5). Then, expression profile of 33685 genes in 18 root samples of Medicago as numerical features, as well as study ID and Arbuscular mycorrhiza type as categorical features, were mined by seven models: RELIEF, UNCERTAINTY, GINI INDEX, Chi Squared, RULE, INFO GAIN, and INFO GAIN RATIO. Batch effect, the major concern in expression meta-analysis, was reduced by three normalization steps: Robust Multi-array Average algorithm, Z-standardization, and quartiling normalization. Expression profiles of roots in response to AM species were collected from four separate studies and were combined by direct merging meta-analysis. We developed a framework for finding the transcriptomic signature of Arbuscular mycorrhiza colonization and its regulating transcription factors in roots of Medicago truncatula.
![reliability workbench fault tree batch analysis reliability workbench fault tree batch analysis](https://i.ytimg.com/vi/p69bIJ8opTE/maxresdefault.jpg)
Unraveling the unified transcriptomic signature of a successful colonization provides a better understanding of symbiosis. Plant root symbiosis with Arbuscular mycorrhizal (AM) fungi improves uptake of water and mineral nutrients, improving plant development under stressful conditions.